Composite

Part:BBa_K1462450:Design

Designed by: Ye Peng   Group: iGEM14_SCUT   (2014-10-10)

pGAL1+GFP+tADH1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1199


Design Notes

This recombinant is designed for test and quantification of GAL1 promoter used in our project.

Figure 1. Test of Gal1 promoter The result shows that the Gal1 promoter works.
Figure 2. Quantification of Gal1 promoter The result shows that the intensity of GFP expressing cells induced by low or high dose of galactose are higher then those induced by the middle doses.



Source

All parts were got from kit.


References